Topical ALA–PDT modifies neutrophils’ chemiluminescence, lymphocytes’ interleukin-1beta secretion and serum level of transforming growth factor beta1 in patients with nonmelanoma skin malignancies: A clinical study

Adamek, Mariusz; Kawczyk-Krupka, Aleksandra; Mostowy, Aleksandra; Czuba, Zenon; Krol, Wojciech; Kasperczyk, Slawomir; Jakobisiak, Marek; Golab, Jakub; Sieron, Aleksander

doi:10.1016/S1572-1000(05)00004-9

« Previous Next »Photodiagnosis and Photodynamic Therapy
Volume 2, Issue 1 , Pages 65-72, March 2005

Topical ALA–PDT modifies neutrophils’ chemiluminescence, lymphocytes’ interleukin-1beta secretion and serum level of transforming growth factor beta1 in patients with nonmelanoma skin malignancies:

A clinical study

Mariusz Adamek, MD, PhD
- Center for Laser Diagnostics and Therapy, Chair and Clinic of Internal Diseases and Physical Medicine, Silesian Medical University, 15 Batory St., PL-41902 Bytom, Poland
- Corresponding author. Tel.: +48 32 786 1616; fax: +48 32 786 1630.
Aleksandra Kawczyk-Krupka
- Center for Laser Diagnostics and Therapy, Chair and Clinic of Internal Diseases and Physical Medicine, Silesian Medical University, 15 Batory St., PL-41902 Bytom, Poland
Aleksandra Mostowy
- Department of Microbiology and Immunology, Silesian Medical University, 19 Jordana St., PL-41808 Zabrze-Rokitnica, Poland
Zenon Czuba
- Department of Microbiology and Immunology, Silesian Medical University, 19 Jordana St., PL-41808 Zabrze-Rokitnica, Poland
Wojciech Krol
- Department of Microbiology and Immunology, Silesian Medical University, 19 Jordana St., PL-41808 Zabrze-Rokitnica, Poland
Slawomir Kasperczyk
- Department of Biochemistry, Silesian Medical University, 19 Jordana St., PL-41808 Zabrze-Rokitnica, Poland
Marek Jakobisiak
- Department of Immunology, Center of Biostructure Research, The Medical University of Warsaw, Chalubinskiego 5, PL-02004 Warsaw, Poland
Jakub Golab
- Department of Immunology, Center of Biostructure Research, The Medical University of Warsaw, Chalubinskiego 5, PL-02004 Warsaw, Poland
Aleksander Sieron
- Center for Laser Diagnostics and Therapy, Chair and Clinic of Internal Diseases and Physical Medicine, Silesian Medical University, 15 Batory St., PL-41902 Bytom, Poland

Summary

Background:

Photodynamic therapy (PDT) has been recognized as a noninvasive therapeutic approach for the effective treatment of tumors. It has been shown in studies conducted on malignant cell lines and various animal tumor models, that the interaction of photosensitizing substances with light leads to the release of cytotoxic substances and stimulates the immune response.

Purpose:

The aim of our study was to analyze the immune system response in patients undergoing photodynamic therapy due to basal cell carcinoma (BCC).

Methods:

Patients with skin malignancies have been treated by 10% delta-aminolevulinic acid (ALA) (Medac GmbH, Wedel, Germany) topically and light from a diode laser. Blood samples were obtained from each patient twice in the same day: before and 4h after photodynamic treatment procedure. In patients’ serum the concentration of transforming growth factor beta1 (TGF-β1) was determined. Additionally the study has been conducted on lymphocytes and granulocytes from peripheral blood. In cell culture supernatants the concentration of interleukin 1beta (IL-1β), interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor alpha (TNFα), the percentile composition of patients’ lymphocytes and the chemiluminescence of neutrophils have been measured.

Results:

We have observed a significant increase (p=0.015) in the intensity of the neutrophil chemiluminescence and significant diminution (p=0.006) of IL-1β concentration in supernatants. Similarly the serum level of TGF-β1 has been significantly decreased (p<0.001).