Photodynamic inactivation of influenza virus with fullerene C60 suspension in allantoic fluid

Zarubaev, Vladimir V.; Belousova, Inna M.; Kiselev, Oleg I.; Piotrovsky, Levon B.; Anfimov, Pavel M.; Krisko, Tatyana C.; Muraviova, Tatyana D.; Rylkov, Vladimir V.; Starodubzev, Andrey M.; Sirotkin, Alexey C.

doi:10.1016/j.pdpdt.2006.08.003

« Previous Next »Photodiagnosis and Photodynamic Therapy
Volume 4, Issue 1 , Pages 31-35, March 2007

Photodynamic inactivation of influenza virus with fullerene C₆₀ suspension in allantoic fluid

Vladimir V. Zarubaev, PhD
- Influenza Research Institute RAMS, 197376 St. Petersburg, Russia
- Corresponding author. Tel.: +7 812 234 6725; fax: +7 812 234 5973.
Inna M. Belousova
- State Optical Institute, 199034 St. Petersburg, Russia
Oleg I. Kiselev
- Influenza Research Institute RAMS, 197376 St. Petersburg, Russia
Levon B. Piotrovsky
- Influenza Research Institute RAMS, 197376 St. Petersburg, Russia
Pavel M. Anfimov
- Influenza Research Institute RAMS, 197376 St. Petersburg, Russia
Tatyana C. Krisko
- State Optical Institute, 199034 St. Petersburg, Russia
Tatyana D. Muraviova
- State Optical Institute, 199034 St. Petersburg, Russia
Vladimir V. Rylkov
- State Optical Institute, 199034 St. Petersburg, Russia
Andrey M. Starodubzev
- State Optical Institute, 199034 St. Petersburg, Russia
Alexey C. Sirotkin
- Influenza Research Institute RAMS, 197376 St. Petersburg, Russia

published online 10 October 2006.

Summary

Background

Viruses are the most dangerous infectious contaminants of human donor blood and blood products. The purpose of the study was to investigate the virus-inactivating properties of fullerene suspension regarding influenza virus in allantoic fluid of chicken embryos.

Methods

Influenza virus was propagated in chicken embryos, water suspension of C₆₀ fullerene was added to the allantoic fluid. The fluid was light-irradiated at constant oxygen flow through the specimen, and the dynamics of virus titer was studied by virus titration in MDCK cells. The morphology of virions was studied by electron microscopy (EM).

Results

Dramatic drop of infectious titer (8 to 1 log10 EID50) of the virus was observed within 2h after start of irradiation. No change of the titers was observed in control specimens without fullerene, or light, or oxygen. EM study revealed numerous defects of virions’ morphology (destruction of outer membrane) leading to the loss of infectious properties of the virus.

Conclusions

Water-insoluble fullerenes may be considered as a prospective way for inactivation of enveloped viruses in biological materials including blood products.

Keywords: Influenza virus, Photoinactivation, Fullerene C₆₀