Photodiagnosis and Photodynamic Therapy
Volume 4, Issue 2 , Pages 106-111, June 2007

Two-photon excitation studies of m-THPC photosensitizer and photodynamic activity in an epithelial cell line

  • M. Atif

      Affiliations

    • Department of Physics, University of Hull, Hull HU6 7RX, UK
    • Department of Physics and Applied Mathematics, Pakistan Institute of Engineering and Applied Sciences, Islamabad, Pakistan
    • ,
  • P.E. Dyer

      Affiliations

    • Department of Physics, University of Hull, Hull HU6 7RX, UK
    • ,
  • T.A. Paget

      Affiliations

    • Department of Biology, University of Hull, Hull HU6 7RX, UK
    • Current address: Medway School of Pharmacy, The Universities of Kent and Greenwich at Medway, Kent ME4 4TB, UK.
    • ,
  • H.V. Snelling, PhD, BSc

      Affiliations

    • Department of Physics, University of Hull, Hull HU6 7RX, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 1482 465690; fax: +44 1482 465606.
    • ,
  • M.R. Stringer

      Affiliations

    • Institute of Microwaves and Photonics, University of Leeds, UK

published online 12 December 2006.

Summary 

A femtosecond laser delivering pulses of wavelength 800nm and 124fs duration at rates of 1kHz has been used to investigate the two-photon excited fluorescence in the photosensitizer m-THPC. The scaling of fluorescence amplitude with laser power and fluorescence sidelight imaging are found to support a predominantly two-photon excitation mechanism. A value for the two-photon cross-section of δ=1.8×10−57m4s is derived by comparing the fluorescence signals excited by wavelengths of 800 and 400nm. Preliminary results demonstrating the two-photon induced PDT activity of m-THPC in an epithelial cell line are also reported.

Keywords: Photodynamic therapy, Femtosecond, Foscan, Two photon

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PII: S1572-1000(06)00147-5

doi:10.1016/j.pdpdt.2006.10.004

Photodiagnosis and Photodynamic Therapy
Volume 4, Issue 2 , Pages 106-111, June 2007

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