Photodiagnosis and Photodynamic Therapy
Volume 4, Issue 2 , Pages 106-111 , June 2007

Two-photon excitation studies of m-THPC photosensitizer and photodynamic activity in an epithelial cell line

  • M. Atif

      Affiliations

    • Department of Physics, University of Hull, Hull HU6 7RX, UK
    • Department of Physics and Applied Mathematics, Pakistan Institute of Engineering and Applied Sciences, Islamabad, Pakistan
    • ,
  • P.E. Dyer

      Affiliations

    • Department of Physics, University of Hull, Hull HU6 7RX, UK
    • ,
  • T.A. Paget

      Affiliations

    • Department of Biology, University of Hull, Hull HU6 7RX, UK
    • Current address: Medway School of Pharmacy, The Universities of Kent and Greenwich at Medway, Kent ME4 4TB, UK.
    • ,
  • H.V. Snelling, PhD, BSc

      Affiliations

    • Department of Physics, University of Hull, Hull HU6 7RX, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 1482 465690; fax: +44 1482 465606.
    • ,
  • M.R. Stringer

      Affiliations

    • Institute of Microwaves and Photonics, University of Leeds, UK

References 

  1. See, for example, the number of papers describing new multiphoton microscopy techniques in Multiphoton Microscopy in the Biomedical Sciences, Periasamy A, So PTC, editors, Proc SPIE 2001;4262.
  2. Hoffman K, Stücker M, Altmeyer P, Teuchner K, Leupold D. Selective femtosecond pulse-excitation of melanin fluorescence in tissue. J Invest Dermatol. 2001;116:629–630
  3. Bhawalkar JD, Kumar JND, Zhao CF, Prasad PN. Two-photon photodynamic therapy. J Clin Laser Med Surg. 1997;15:201–204
  4. Fischer WG, Partridge WP, Dees C, Wachter EA. Simultaneous two-photon activation of Type-I photodynamic agents. Photochem Photobiol. 1997;66:141–155
  5. Karotki A, Kruk M, Drobizhev M, Rebane A, Nickel E, Sprangler CW. Efficient singlet oxygen generation upon two-photon excitation of new porphyrin with enhanced non-linear absorption. IEEE J Selected Topics Quant Electron. 2001;7:971–975
  6. Fournier M, Pepin C, Houde D, Ouellet R, van-Lier JE. Ultrafast studies of the excited state dynamics of copper and nickel phthalocyanine tetrasulfonates: potential sensitizers for the two-photon photodynamic therapy of tumors. Photochem Photobiol Sci. 2003;3:120–126
  7. Schneider M, Graschew G, Roelofs TA, et al. Multiphoton excitation and photodynamic activity of macromolecular derivatized mTHPC. Proc SPIE. 2000;3909:60–65
  8. Goyan RL, Cramb DT. Near-infrared two-photon excitation of protoporphyrin. IX. Photodynamics and photoproduct generation. Photochem Photobiol. 2000;72:821–827
  9. Samkoe KS, Cramb DT. Application of an ex ovo chicken chorioallantoic membrane model for two-photon excitation photodynamic therapy of age-related macular degeneration. J Biomed Opt. 2003;8:410–417
  10. Liu J, Zhao YW, Zhao JQ, et al. Two-photon excitation studies of hypocrellins for photodynamic therapy. J Photocem Photobiol B. 2002;68:156–164
  11. Song JM, Inoue T, Kawazumi H, Ogawa T. Determination of two photon absorption cross-section of fluorescein using a mode locked titanium sapphire laser. Anal Sci. 1999;15:601–603
  12. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxity assays. J Immunol Meth. 1983;16:55–63
  13. Dyart JS, Patterson MS, Farrell TJ, Singh G. Relationship between mTHPC fluorescence photobleaching and cell viability during in vitro photodynamic treatment of DP16 cells. Photochem Photo-Biol. 2002;75:289–295
  14. Lakowicz JR. Principles of fluorescence spectroscopy. 2nd ed.. New York: Kluwer Academic/Plenum; 1999;
  15. Atif M, Stringer MR, Cruse-Sawyer JE, Brown SC. Fluence-rate effects upon m-THPC photobleaching in a formalin-fixed cell system. Photodiag Photodyn Therapy. 2004;1:173–180
  16. Drobizhhev M, Stepanenko Y, Dzenis Y, et al. Understanding strong two-photon absorption in π -conjugated porphyrin dimers via double-resonance enhancement in a three-level model. J Am Chem Soc. 2004;126:15352–15353

PII: S1572-1000(06)00147-5

doi: 10.1016/j.pdpdt.2006.10.004

Photodiagnosis and Photodynamic Therapy
Volume 4, Issue 2 , Pages 106-111 , June 2007

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