Optical biopsy using spectral camera in BCC and oral leukoplakia

Sieron, A.; Gibinski, P.; Pustelny, T.; Kwiatek, S.; Opilski, Z.; Kawczyk-Krupka, A.; Woznica, T.; Maciak, E.; Kubica, W.; Urbanczyk, M.; Latos, W.

doi:10.1016/j.pdpdt.2008.10.001

« Previous Next »Photodiagnosis and Photodynamic Therapy
Volume 5, Issue 4 , Pages 271-275, December 2008

Optical biopsy using spectral camera in BCC and oral leukoplakia☆

A. Sieron
- Department of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, 15 Batorego St., Bytom, Poland
P. Gibinski
- Institute of Medical Technology and Equipment ITAM, 118 F. Roosevelt St., 41-800 Zabrze, Poland
- Tel.: +48 32 271 60 13; fax: +40 32 276 56 08.
T. Pustelny
- Faculty of Mathematics and Physics - Silesian University of Technology, 2 Krzywoustego St., 44-100 Gliwice, Poland
- Tel.: +48 32 237 22 16; fax: +48 32 237 22 16.
S. Kwiatek
- Department of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, 15 Batorego St., Bytom, Poland
Z. Opilski
- Faculty of Mathematics and Physics - Silesian University of Technology, 2 Krzywoustego St., 44-100 Gliwice, Poland
- Tel.: +48 32 237 22 16; fax: +48 32 237 22 16.
A. Kawczyk-Krupka, MD
- Department of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, 15 Batorego St., Bytom, Poland
- Corresponding author. Tel.: +48 32 786 16 30.
T. Woznica
- Institute of Medical Technology and Equipment ITAM, 118 F. Roosevelt St., 41-800 Zabrze, Poland
- Tel.: +48 32 271 60 13; fax: +40 32 276 56 08.
E. Maciak
- Faculty of Mathematics and Physics - Silesian University of Technology, 2 Krzywoustego St., 44-100 Gliwice, Poland
- Tel.: +48 32 237 22 16; fax: +48 32 237 22 16.
W. Kubica
- Institute of Medical Technology and Equipment ITAM, 118 F. Roosevelt St., 41-800 Zabrze, Poland
- Tel.: +48 32 271 60 13; fax: +40 32 276 56 08.
M. Urbanczyk
- Faculty of Mathematics and Physics - Silesian University of Technology, 2 Krzywoustego St., 44-100 Gliwice, Poland
- Tel.: +48 32 237 22 16; fax: +48 32 237 22 16.
W. Latos
- Department of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, 15 Batorego St., Bytom, Poland

published online 10 November 2008.

Summary

Basal cell carcinoma is one of the most common neoplasmatic diseases of the skin. Oral leukoplakia is precancerous stage. Early diagnosis of both diseases gives very good treatment results. Fluorescence spectroscopy is a new modality of diagnostics using excitation of absorbed PPIX in tumor. Spectroscopy is an objective method showing concentration and emission spectrum of protoporphyrin IX. In fluorescence spectroscopy with PPIX we used diode light 405nm to induce fluorescence and observed significant emission peak at 630nm. Fluorescence spectroscopy is based on searching of emission peaks of photosensitizer in areas of BCC and oral leukoplakia. In our study we used spectral camera measuring spectrum from pictures taken in different wavelengths between 570nm and 650nm. The pictures taken with this camera were compared with the pictures captured with Xillix Onco Life system. 20 patients with histopathologically confirmed BCC and 10 with oral leukoplakia were examinated with spectral camera and with Xillix Onco Life system 8h after local instillation of delta aminolevulinic acid. Our spectral camera made pictures with different filters for wavelength between 570nm and 650nm showing highest fluorescence intensity in 630nm. Captured spectral lines were compared with NCV (numerical color value) from Xillix Onco Life. All of spectral lines taken from neoplasmatic tissues were compared with spectral lines taken from healthy tissues in symmetrical areas of body. Results were statistically processed. All of spectral lines taken from neoplasmatic tissues showed significant peak at 630nm in the area of BCC and oral leukoplakia in all of the examinated patients. Spectral lines taken from healthy tissues were significantly different. There was no peak in 630nm and fluorescence intensity was decreasing from 570nm to 630nm. Intensity of fluorescence in 630nm was in high correlation with NCV from Xillix Onco Life.