Photodiagnosis and Photodynamic Therapy
Volume 6, Issue 1 , Pages 46-51, March 2009

Interactions of protoporphyrin IX and its derivatives with benzodiazepine receptor

Institute of Optoelectronics, Military Academy of Technology, Gen. S. Kaliskiego 2 Str., 00-908 Warsaw, Poland

published online 26 February 2009.

Summary 

The aim of this study was to determine binding affinities of the Peripheral benzodiazepine receptor (PBR) with protoporphyrin IX, haematoporphyrin (Hp), and two arginized derivatives: Hp(Arg)2 and PP(Arg)2. The quenching of protein fluorescence was used to measure association constants during titration of PBR solution with PP derivatives in a function of concentration. The experimental data was fitted to theoretical curves, assuming strong and weak binding model with one binding site. As a result of this early study affinity constants were determined. The highest affinity toward PBR showed PPIX and Hp, Hp(Arg)2, PP(Arg)2 lower, respectively. Changes in pH of protein solution resulted in decrease of association constants in nearly all examined derivatives.

Keywords: Protoporphyrin IX, Peripheral benzodiazepine receptor (PBR), Association constant

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PII: S1572-1000(09)00005-2

doi:10.1016/j.pdpdt.2009.01.004

Photodiagnosis and Photodynamic Therapy
Volume 6, Issue 1 , Pages 46-51, March 2009

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