Photodiagnosis and Photodynamic Therapy
Volume 6, Issue 2 , Pages 117-121, June 2009

Photo-toxic effects of 809-nm diode laser and indocyanine green on MDA-MB231 breast cancer cells

  • Ozguncem Bozkulak

      Affiliations

    • Bogazici University, Institute of Biomedical Engineering, 34342, Bebek, Istanbul, Turkey
    • Tel.: +90 212 359 75 76; fax: +90 212 257 50 30.
    • ,
  • Rezan Fahrioglu Yamaci, PhD

      Affiliations

    • Bogazici University, Department of Molecular Biology and Genetics, 34342, Bebek, Istanbul, Turkey
    • Tel.: +90 212 359 66 27; fax: +90 212 287 24 68.
    • ,
  • Ozgur Tabakoglu

      Affiliations

    • Bogazici University, Institute of Biomedical Engineering, 34342, Bebek, Istanbul, Turkey
    • Tel.: +90 212 359 75 76; fax: +90 212 257 50 30.
    • ,
  • Murat Gulsoy, PhD

      Affiliations

    • Bogazici University, Institute of Biomedical Engineering, 34342, Bebek, Istanbul, Turkey
    • Corresponding Author InformationCorresponding author. Tel.: +90 212 359 70 26; fax: +90 212 257 50 30.

published online 31 July 2009.

Summary 

Photodynamic therapy (PDT), due to its positive outcomes in clinical applications, easiness of practice and few side effects, is a good candidate for an efficient treatment of cancer. Indocyanine green (ICG), a water-soluble, anionic tricarbocyanine and non-toxic molecule is a promising photosensitive agent for PDT applications on tumor cells. ICG exhibits strong maximum absorption at around 805nm which will be an advantage for its use in PDT; light at that wavelength can be used to treat deeper tumors. In this study the inhibitory growth effects of ICG-PDT on MDA-MB231 human breast cancer cells were investigated in a time course experiment. Cells were irradiated with a continuous wave diode laser (λ=809nm, 60mW, 24Jcm−2). Cell viability was measured by MTT assay 0, 3, 6, 9, 12, 24 and 48h after light irradiation. The results showed that ICG-PDT application exerted its photo-oxidative effect on MDA-MB231 breast cancer cells immediately. Relative cell viability was determined throughout the 48h time course, and a consistent decrease was observed after ICG-PDT applications. In conclusion, ICG when used in combination with near-infrared light showed a very fast (within 3h) and persistent (up to 48h) photo-toxic effect on MDA-MB231 human breast cancer cells.

Keywords: Indocyanine green, 809-nm diode laser, Photodynamic therapy, MDA-MB231

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PII: S1572-1000(09)00060-X

doi:10.1016/j.pdpdt.2009.07.001

Photodiagnosis and Photodynamic Therapy
Volume 6, Issue 2 , Pages 117-121, June 2009

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